Assignment 18: Annotated Bibliography 2

Source:

Quiett, Jilian. PGX & MDX Department Handbook. Aeon Global Health, 2017.

Summary:

A concrete detailing of the PGX (Pharmacogenomics) and MDX (Women's Health) protocol for Aeon Global Health. A guidebook for all protocol within the PGX lab, complete with pictures, time guidelines and requirements to begin certain protocol. Begins with Sample Accessioning, and walks the reader through each process past Open Array Analysis.

Quotes:

"RNase P is a method used to quantify gDNA. Each patien sample, positive control and NTC will be run in a triplicate."

"The MagMAX Express magnetic particle processor utilizes permanent magnetic rods and disposable tip combs with independent movement control. When the magnetic rod- sheathed inside the tip comb -is lowered into solution, magnetic beads collect at the bottom of the tip comb. The tip comb can then be positioned in a different well or plate and the beads released by moving the magnetic rods out of the tip comb. The tip comb also mixes the reagents and magnetic beads. The effectiveness of bead collection and transfer leads to superior washing, elution efficiency, and rapid procession"

Source Evaluation:

-Written by Jilian Quiett, Pharmacogenomic Laboratory Manager and Scientist in the field of Pharmacogenomics.

-Up-to-Date sources and current, in-effect protocol

Source is Reliable

Reflection:

Source is the basis of my experiment, as I will be walking through the book with said experiment. With the book, and prior knowledge of the protocols discussed in the book, my experiment should go off with out a hitch.